High-Performance Liquid Chromatography (HPLC)
Agilent 1260 Infinity II Analytical LC System
The Agilent 1260 Infinity II is a fully modular, high-pressure liquid chromatography platform used for the separation, identification, and quantification of non-volatile, thermally labile, polar, and high-molecular-weight compounds that are not amenable to gas chromatography. The system's quaternary/binary pump delivers precise, low-pulsation solvent gradients at pressures up to 600 bar, while the thermostatted multicolumn compartment and programmable autosampler ensure highly reproducible retention times across long analytical sequences.
Coupled with a UV-Vis diode-array detection module, the system provides multi-wavelength spectral confirmation alongside quantitative analysis and can be configured with a broad range of column chemistries — reversed-phase (C18/C8), normal-phase, HILIC, ion-exchange, and size-exclusion — to address virtually any liquid-phase separation challenge.
Analytical Capabilities & Compatible Sample Types
Applicable to non-volatile, polar, ionic, thermally unstable, and high-molecular-weight compounds across a wide polarity range.
- Pharmaceutical Compounds — APIs, degradation products, and process-related impurities.
- Food Components — — Preservatives, sweeteners, vitamins, organic acids, and mycotoxins.
- Environmental Contaminants — Pesticides, pharmaceuticals and personal-care products (PPCPs) in water.
- Natural Products & Dyes — Plant extracts, pigments, and synthetic/natural colorants.
- Polymers & Macromolecules — Molecular weight distribution via size-exclusion chromatography (SEC/GPC).
Applications
Academia & Research
Isolation and purity assessment of synthesized and natural compounds.
Undergraduate and graduate analytical/pharmaceutical chemistry laboratory instruction.
Method development and validation studies for thesis and funded research.
Biomolecule and polymer characterization for materials research.
Industry & Commercial Services
Pharmaceutical industry — API assay, dissolution testing, and stability studies (ICH-aligned).
Food and beverage industry — additive, preservative, and mycotoxin quantification.
Agrochemical sector — pesticide and herbicide residue analysis.
Cosmetics industry — active ingredient and preservative quantification.
